# Pathway Enrichment The enrichment analysis identifies if the input set of genes from the report significantly overlaps with annotated gene sets and pathways. {% hint style="info" %} In order to run a pathway enrichment, the unbiased concept used within the report must contain Genes {% endhint %} \ Step 1: Create a graph model where one of your concepts contains genes. Detailed instructions on how to do this [can be found here](https://docs.biobox.io/guide/how-to/create-prioritization-graph-models). Step 2: Create a report using the model where the unbiased concept is "Gene". Step 3: Open the report and select the "Pathway Enrichment" tab. {% embed url="" %} By default the first 1500 genes returned in the report will be used for the pathway enrichment. To adjust this number select "filter" and you can update the total number of genes included in the pathway enrichment. For example setting the start to 0 and end to 100 will include the first 100 genes. \ \ Use the database input to select from numerous databases. Click on a pathway to view the genes from your report that are involved in the selected pathway. --- # Agent Instructions: Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. Perform an HTTP GET request on the current page URL with the `ask` query parameter: ``` GET https://docs.biobox.io/guide/how-to/create-prioritization-graph-models/pathway-enrichment.md?ask= ``` The question should be specific, self-contained, and written in natural language. The response will contain a direct answer to the question and relevant excerpts and sources from the documentation. Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.